EK finansētie resursi / EC funded resources

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Browsing EK finansētie resursi / EC funded resources by Author "Erts, Donats"

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    Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods
    (2019-04-15) Viter, Roman; Savchuk, Maryna; Starodub, Nickolay; Balevicius, Zigmas; Tumenas, Saulius; Ramanaviciene, Almira; Jevdokimovs, Daniels; Erts, Donats; Iatsunskyi, Igor; Ramanavicius, Arunas
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    Porous Aluminium Oxide Coating for the Development of Spectroscopic Ellipsometry Based Biosensor: Evaluation of Human Serum Albumin Adsorption
    (MDPI, 2020-10-23) Maciulis, Vincentas; Malinovskis, Uldis; Erts, Donats; Ramanavicius, Arunas; Ramanaviciene, Almira; Balevicius, Saulius; Juciute, Silvija; Plikusiene, Ieva
    An electrochemically synthesised porous anodic aluminium oxide (pAAO) layer has been analysed by means of spectroscopic ellipsometry. The determined thickness of the formed pAAO layer obtained from spectroscopic ellipsometry measurements and modelling was 322.75 ± 0.12 nm. The radius of the nanopores estimated from SEM images was 39 ± 5 nm and the distance between nanopores was 107 ± 6 nm. The investigation of human serum albumin (HSA) adsorption on the pAAO coating showed that: (i) the protein concentration inside nanopores, depending on exposure time, approximately was from 200 up to 600 times higher than that determined in buffer solution; (ii) the initial phase of the adsorption process is slow (3.23 mg·cm−3·min−1) in comparison with the protein desorption rate (21.2 mg·cm−3·min−1) by means of pAAO layer washing; (iii) conventional washing with PBS solution and deionised water does not completely remove HSA molecules from pAAO pores and, therefore, the HSA concentration inside nanopores after 16 h of washing still remains almost 100 times higher than that present in PBS solution. Thus, due to such binding ability, HSA can be successfully used for the blocking of the remaining free surface, which is applied for the reduction in non-specific binding after the immobilisation of biorecognition molecules on the pAAO surface. It was determined that some desorption of HSA molecules from the pAAO layer occurred during the sensor’s surface washing step; however, HSA concentration inside the nanopores still remained rather high. These results recommend the continued application of pAAO in the development of biosensors.
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    Visible Photoluminescence of Variable-Length Zinc Oxide Nanorods Embedded in Porous Anodic Alumina Template for Biosensor Applications
    (MDPI, 2021-06-24) Malinovskis, Uldis; Dutovs, Aleksandrs; Poplausks, Raimonds; Jevdokimovs, Daniels; Graniel, Octavio; Bechelany, Mikhael; Muiznieks, Indrikis; Erts, Donats; Prikulis, Juris
    Zinc oxide (ZnO) and porous anodic aluminum oxide (PAAO) are technologically important materials, rich with features that are of interest in optical applications, for example, in light-emitting and sensing devices. Here, we present synthesis method of aligned ZnO nanorods (NR) with 40 nm diameter and variable length in 150 to 500 nm range obtained by atomic layer deposition (ALD) of ZnO in pores of continuously variable thickness PAAO. The relative intensity of yellow (1.99 eV), green (2.35 eV), and blue (2.82 eV) photoluminescence (PL) components originating from the different types of defects, varied with non-monotonic dependency on the composite film thickness with a Fabry–Pérot like modulation. The intensity variation of any individual PL component correlated well with anti-reflective properties of ZnO NR–PAAO composite film at the peak wavelength of the particular PL component. This provides a route for selective enhancement or suppression of color components of hybrid fluorescent emitters by tuning only geometric parameters, with potential use in imaging and other optical devices. As an application example we tested the composite film for sensing of vascular endothelial growth factor (VEGF) using a widely accessible fluorescence microscopy setup. The intensity of the yellow and green PL components reduced in response to increased VEGF concentrations, whereas blue component remained invariant.
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    Zinc oxide nanorod based immunosensing platform for the determination of human leukemic cells
    (2019-08-01) Tamashevski, Alexander; Harmaza, Yuliya; Viter, Roman; Jevdokimovs, Daniels; Poplausks, Raimonds; Slobozhanina, Ekaterina; Mikoliunaite, Lina; Erts, Donats; Ramanaviciene, Almira; Ramanavicius, Arunas