Biomedical optical systems and models can be easily validated by the use of tissue-simulating phantoms. They can
consist of water-based turbid media which often include inks (India ink and molecular dyes) as absorbers. Optical
stability of commonly exploited inks under the influence of light, pH changes and the addition of TiO2 and surfactant,
was studied. We found that the exposure to ultraviolet and visible light can crucially affect the absorption properties of
molecular dyes. On average, absorption peaks decreased by 47.3% in 150 exposure hours. Furthermore, dilution can
affect ink’s pH and by that, its decay rate under light exposure. When TiO2 was added to the phantoms, all molecular
dyes decayed rapidly. Photocatalytic nature of TiO2 can be partially avoided by selecting TiO2 with surface and crystal
structure modification. Surfactant, normally present in the phantoms with polystyrene spheres, can cause absorption peak
shifts up to 20 nm and amplitude changes of 29.6%. Therefore, it is crucial to test the optical stability of inks in the
presented manner before their exploitation in water-based phantoms.