Trimetillizīna kvantitatīva noteikšana bioloģiskos paraugos ar šķidrumu hromatogfāfijas-masspektrometrijas metodi
Date
2012
Authors
Sevostjanovs, Eduards
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Publisher
Latvijas Universitāte
Abstract
Darbā apkopota literatūra par trimetillizīna kvantitatīvās analīzes iespējām ar šķidrumu hromatogrāfijas metodēm. Izstrādāta un validēta jauna šķidrumu hromatogrāfijas – tandēmmasspektrometrijas metode trimetillizīna kvantitatīvai noteikšanai bioloģiskos paraugos, izmantojot derivatizāciju ar 6-aminohinolil-N-hidroksisukcīnimidilkarbamātu. Izstrādātā metode sekmīgi pielietota karnitīna biosintēzē iesaistīto savienojumu koncentrāciju mērījumiem žurku asins plazmā un audu homogenātos.
The theoretical part of this thesis contains a literature review of methods for quantitative analysis of trimethyllysine by liquid chromatography. A new liquid chromatography – tandem mass spectrometry method was developed and validated for quantitative determination of trimethyllysine in biological samples by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. The developed method was succesfully employed in the study of carnitine biosynthesis intermediates in rat plazma and tissue homogenates.
The theoretical part of this thesis contains a literature review of methods for quantitative analysis of trimethyllysine by liquid chromatography. A new liquid chromatography – tandem mass spectrometry method was developed and validated for quantitative determination of trimethyllysine in biological samples by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. The developed method was succesfully employed in the study of carnitine biosynthesis intermediates in rat plazma and tissue homogenates.
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