1,4-Dihidropiridīnu atvasinājumu nanodaļiņu toksiskuma novērtējums mikroorganismos
Date
2017
Authors
Ķibilda, Signe
Journal Title
Journal ISSN
Volume Title
Publisher
Latvijas Universitāte
Abstract
1,4 Dihidropiridīnu (1,4 DHP) atvasinājumi ir plaša organisko vielu grupa, kurā arvien tiek sintezēti jauni savienojumi ar plašām pielietojuma iespējām. Jauno savienojumu toksicitātes raksturojums ir nepieciešams, lai paredzētu to pielietojuma iespējas un novērtētu to iedarbību uz vidi un dzīvajiem organismiem. Darbā pielāgota universāla un efektīva atšķaidījumu metode, lai noteiktu deviņu katjono amfifilo 1,4 DHP atvasinājumu toksicitātes robežkoncentrācijas sešām baktērijām un vienam raugam. Papildus noteikta 1,4 DHP atvasinājumu ietekme uz iekššūnas ATP daudzumu, skābekļa patēriņa ātrumu un etīdija bromīda transportu šūnās. Noskaidrojot toksicitātes robežkoncentrācijas un gūstot ieskatu potenciālajos toksicitātes faktoros, secināts, ka toksicitāti veido vairāku faktoru mijiedarbība.
1,4-Dihydropyridine (1,4-DHP) derivatives is an increasingly diverse group of organic compounds with a wide range of possible uses. Toxicity assessment of these novel compounds is necessary in order to predict possible applications and determine the impact on living organisms and environment. In this study, a universal and efficient microdilution method was adjusted for use in determination of borderline toxicity concentrations for nine cationic amphiphilic 1,4 DHP derivatives in six species of bacteria and one yeast. In addition, toxic effects were examined in more detail by measuring intracellular ATP amount, oxygen consumption rate and ethidium bromide transport in treated cells. Estimations of borderline toxicity and gained insights about toxicity factors allowed to conclude that 1,4 DHP toxicity is a combination of multiple factors.
1,4-Dihydropyridine (1,4-DHP) derivatives is an increasingly diverse group of organic compounds with a wide range of possible uses. Toxicity assessment of these novel compounds is necessary in order to predict possible applications and determine the impact on living organisms and environment. In this study, a universal and efficient microdilution method was adjusted for use in determination of borderline toxicity concentrations for nine cationic amphiphilic 1,4 DHP derivatives in six species of bacteria and one yeast. In addition, toxic effects were examined in more detail by measuring intracellular ATP amount, oxygen consumption rate and ethidium bromide transport in treated cells. Estimations of borderline toxicity and gained insights about toxicity factors allowed to conclude that 1,4 DHP toxicity is a combination of multiple factors.
Description
Keywords
Bioloģija , 1,4-dihidropiridīna atvasinājumi , nanodaļiņas , toksicitātes novērtējums , etīdija bromīda transports , ATP