1,4-Dihidropiridīnu log D vērtības noteikšana izmantojot augsti efektīvas šķidruma hromatogrāfijas metodi
Date
2011
Authors
Koliškina, Olga
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Publisher
Latvijas Universitāte
Abstract
Darbā analizēti literatūras avoti par organisko savienojumu log P (log D) vērtību noteikšanu. Izmantojot rezultātus iegūtos iepriekšējos kursa darbos tika izvēlēti eksperimentāli apstākļi 1,4-dihidropiridīnu (DHP) log D7,4 vērtības noteikšanai ar augsti efektīvās šķidrumu hromatogrāfijas (AEŠH) metodi un pārbaudīta šo apstākļu pielietojamība uz 15 jaunsintezētiem DHP rindas savienojumiem. Ar ekstrakcijas metodi jaunsintezētiem DHP eksperimentāli tika noteikti sadalīšanās koeficienti sistēmā n-oktanols/0,08M TRIS buferšķīdums (pH 7,4) un rezultāti salīdzināti gan ar vērtībām noteiktām ar AEŠH, gan ar log P vērtībām iegūtām ar aprēķinu metodēm. Atrasta laba korelācija (R2 ~ 0,97) starp log D7,4 vērtībām noteiktām ar ekstrakcijas un AEŠH metodi.
Literature about determination of log P (log D) is reviewed. Using obtained in previous course work results there are selected experimental conditions for 1,4-dihydropyridine (DHP) log D7.4 values determination by high performance liquid chromatography (HPLC) method and tested the usability of these conditions, using 15 new DHP line compounds. Experimental partition coefficients was obtained for 15 new DHP’s by shake-flask method in system n-octanol/0.08M TRIS (pH 7.4) and results was compared with both the values obtained by HPLC and the log P obtained by calculation method. Found a good correlation (R2 ~ 0.97) between the log D7 .4 values determined by the shake-flask and HPLC methods.
Literature about determination of log P (log D) is reviewed. Using obtained in previous course work results there are selected experimental conditions for 1,4-dihydropyridine (DHP) log D7.4 values determination by high performance liquid chromatography (HPLC) method and tested the usability of these conditions, using 15 new DHP line compounds. Experimental partition coefficients was obtained for 15 new DHP’s by shake-flask method in system n-octanol/0.08M TRIS (pH 7.4) and results was compared with both the values obtained by HPLC and the log P obtained by calculation method. Found a good correlation (R2 ~ 0.97) between the log D7 .4 values determined by the shake-flask and HPLC methods.
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