Lung on a Chip Development from Off-Stoichiometry Thiol–Ene Polymer

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dc.contributor.authorRimsa, Roberts
dc.contributor.authorGalvanovskis, Artis
dc.contributor.authorPlume, Janis
dc.contributor.authorRumnieks, Felikss
dc.contributor.authorGrindulis, Karlis
dc.contributor.authorPaidere, Gunita
dc.contributor.authorErentraute, Sintija
dc.contributor.authorMozolevskis, Gatis
dc.contributor.authorAbols, Arturs
dc.date.accessioned2021-08-26T14:37:19Z
dc.date.available2021-08-26T14:37:19Z
dc.date.issued2021
dc.descriptionInstitute of Solid-State Physics, University of Latvia as the Center of Excellence has received funding from the European Union’s Horizon 2020 Framework Programme H2020-WIDESPREAD-01-2016-2017-TeamingPhase2 under grant agreement No. 739508, project CAMART2. Finally, we would like to thank Biol. Kaspars Tars from Latvian Biomedical research and study center for giving us the opportunity to participate in this consortium and contribute to Latvian scientists’ effort in response to the COVID-19 pandemic.en_US
dc.description.abstractCurrent in vitro models have significant limitations for new respiratory disease research and rapid drug repurposing. Lung on a chip (LOAC) technology offers a potential solution to these problems. However, these devices typically are fabricated from polydimethylsiloxane (PDMS), which has small hydrophobic molecule absorption, which hinders the application of this technology in drug repurposing for respiratory diseases. Off-stoichiometry thiol–ene (OSTE) is a promising alternative material class to PDMS. Therefore, this study aimed to test OSTE as an alternative material for LOAC prototype development and compare it to PDMS. We tested OSTE material for light transmission, small molecule absorption, inhibition of enzymatic reactions, membrane particle, and fluorescent dye absorption. Next, we microfabricated LOAC devices from PDMS and OSTE, functionalized with human umbilical vein endothelial cell (HUVEC) and A549 cell lines, and analyzed them with immunofluorescence. We demonstrated that compared to PDMS, OSTE has similar absorption of membrane particles and effect on enzymatic reactions, significantly lower small molecule absorption, and lower light transmission. Consequently, the immunofluorescence of OSTE LOAC was significantly impaired by OSTE optical properties. In conclusion, OSTE is a promising material for LOAC, but optical issues should be addressed in future LOAC prototypes to benefit from the material properties.--//--This work is licensed under a CC BY 4.0 license.en_US
dc.description.sponsorshipThis research was funded by project Nr. VPP-COVID-2020/1-0014 awarded by Latvian Council of Scienceen_US
dc.identifier.doi10.3390/mi12050546
dc.identifier.issn2072-666X
dc.identifier.urihttps://www.mdpi.com/2072-666X/12/5/546
dc.identifier.urihttps://dspace.lu.lv/dspace/handle/7/56489
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/739508/EU/Centre of Advanced Material Research and Technology Transfer/CAMART²en_US
dc.relation.ispartofseriesMicromachines;12 (5); 546
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectlung on a chipen_US
dc.subjectorgan on a chipen_US
dc.subjectoff-stoichiometry thiol–eneen_US
dc.subjectPDMSen_US
dc.subjectResearch Subject Categories::NATURAL SCIENCES:Physicsen_US
dc.titleLung on a Chip Development from Off-Stoichiometry Thiol–Ene Polymeren_US
dc.typeinfo:eu-repo/semantics/articleen_US
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