Peļu aktivitātes izmaiņas atklātā lauka testā pēc insulta modelēšanas
Date
2020
Authors
Ābola, Agate
Journal Title
Journal ISSN
Volume Title
Publisher
Latvijas Universitāte
Abstract
Išēmisks insults var izraisīt gan neiroloģiskas izmaiņas, tai skaitā sensorimotorās funkcijas un izziņas traucējumus, gan uzvedības izmaiņas. Atklātā lauka tests tiek plaši izmantots, lai novērtētu un analizētu peļu spontāno jeb vispārējo lokomotoro aktivitāti. Bakalaura darba mērķis ir izvērtēt peļu aktivitāti sešus mēnešus pēc insulta modelēšanas, izmantojot atklātā lauka testu. Iegūtie rezultāti rāda, ka Stroke grupas peles uzrāda ievērojami lielāku aktivitāti, griežoties pretpulksteņrādītāja virzienā, salīdzinot gan ar Naive, gan ar Sham grupas pelēm, kas norāda uz insulta bojājumu kontralaterālajā pusē. Naive grupas pelēm ievērojami samazinās aktivitāte, salīdzinot ar pirmo testēšanas reizi, turpretī Sham un Stroke grupas peles neuzrāda tik būtisku aktivitāšu samazināšanos sešu mēnešu periodā.
Ischemic stroke can cause both neurological changes, including sensory motor functions and cognitive impairment, and behavioural changes, such as anxiety and depression. The open field test is widely used to assess and analyse changes in spontaneous or general locomotor activity in mice. The aim of the bachelor’s thesis was to evaluate the activity of mice 6 months after the modelling of stroke using an open field test. The results show that Stroke group mice show significantly more activity when turning counter clockwise compared to both Naive and Sham group mice, indicating stroke damage on the contralateral side. Mice in the Naive group showed a significant reduction in activity compared to the first test, whereas mice in the Sham and Stroke groups did not show such a significant reduction in activity over a six-month period.
Ischemic stroke can cause both neurological changes, including sensory motor functions and cognitive impairment, and behavioural changes, such as anxiety and depression. The open field test is widely used to assess and analyse changes in spontaneous or general locomotor activity in mice. The aim of the bachelor’s thesis was to evaluate the activity of mice 6 months after the modelling of stroke using an open field test. The results show that Stroke group mice show significantly more activity when turning counter clockwise compared to both Naive and Sham group mice, indicating stroke damage on the contralateral side. Mice in the Naive group showed a significant reduction in activity compared to the first test, whereas mice in the Sham and Stroke groups did not show such a significant reduction in activity over a six-month period.
Description
Keywords
Farmācija , insults , peles , lokomotorā aktivitāte , VCAo , atklātā lauka tests