Triciklisko 1,4-dihidropiridīna atvasinājumu un cilvēka telomērisko guanīna kvadrupleksu mijiedarbība
Date
2020
Authors
Pūliņa-Cine, Madara
Journal Title
Journal ISSN
Volume Title
Publisher
Latvijas Universitāte
Abstract
Pēdējos gados tiek pievērsta lielāka uzmanība savienojumiem, kuriem piemīt spēja mijiedarboties ar netipiskām DNS struktūrām – guanīna kvadrupleksiem. Šī pētījuma darba mērķis bija izpētīt triciklisko 1,4-dihidropiridīna atvasinājumu (PP-544-NH4, PP-544-Na, PP-150-Na un B-5-Na) spēju mijiedarboties ar telomēras guanīna antiparalēlām un hibrīdformas kvadrupleksiem, kā arī spēju mijiedarboties ar vienpavediena DNS formu. Rezultātu iegūšanai tika izmantotas spektroskopijas metodes. Lielākajai daļai savienojumu tika novērota mijiedarbība ar telomēru-guanīna kvadrupleksu antiparalēlo un hibrīda konformāciju, kā arī ar vienpavadiena DNS formu, tomēr ar dažadu intensitāti. B-5-Na parādīja vislielāko afinitāti telomēru-guanīna kvadrupleksu hibrīdformai. Kamēr citi analizējamie savienojumi intensīvāk mijiedarbojās ar telomēru-guanīna kvadrupleksu antiparalēlo struktūru
There has been done more research on the compounds that have interaction with non-typical DNA structures – guanine quadruplexes in the past years. In this bachelor’s thesis, interaction between four derivates of 1,4-dihydropiridine (PP-544-NH4, PP-544-Na, PP-150-Na and B-5-Na) and antiparallel and hybrid formation of telomeric guanine quadruplexes were studied. Spectroscopic methods have been used to determine and compare binding of derivates of 1,4-dihydropiridines and DNA. Experimental part has proven most of the compuonds have a binding ability to antiparallel and hybrid formation of telomeric guanine quadruplexes as well as single stranded DNA structure. B-5-Na showed the most affinity to hybrid formation of telomeric guanine quadruplexes. Although other analysed compounds more intensively interacted with antiparallel formation of telomeric guanine quadruplexes
There has been done more research on the compounds that have interaction with non-typical DNA structures – guanine quadruplexes in the past years. In this bachelor’s thesis, interaction between four derivates of 1,4-dihydropiridine (PP-544-NH4, PP-544-Na, PP-150-Na and B-5-Na) and antiparallel and hybrid formation of telomeric guanine quadruplexes were studied. Spectroscopic methods have been used to determine and compare binding of derivates of 1,4-dihydropiridines and DNA. Experimental part has proven most of the compuonds have a binding ability to antiparallel and hybrid formation of telomeric guanine quadruplexes as well as single stranded DNA structure. B-5-Na showed the most affinity to hybrid formation of telomeric guanine quadruplexes. Although other analysed compounds more intensively interacted with antiparallel formation of telomeric guanine quadruplexes
Description
Keywords
Farmācija , 1,4-DIHIDROPIRIDĪNA ATVASINĀJUMI , UV/VIS SPEKTROFOTOMETRIJA , TELOMĒRAS GUANĪNA KVADRUPLEKSI , DERIVATES OF 1,4-DIHYDROPIRIDINE , UV/VIS SPECTROPHOTOMETRY