Glikozes transportieru un slāpekļa oksīda produkcijas izmaiņas cukura diabēta komplikāciju patoģenēzē un to farmakoloģiskās korekcijas iespējas
Date
2014
Authors
Sokolovska, Jeļizaveta
Journal Title
Journal ISSN
Volume Title
Publisher
Latvijas Universitāte
Abstract
Darba mērķis bija izpētīt slāpekļa oksīda metabolisma un glikozes transportieru ekspresijas izmaiņu patoģenētisko nozīmi cukura diabēta un tā komplikāciju attīstībā un pārbaudīt mildronāta un dažu 1,4-dihidropiridīnu spēju normalizēt tās. Rezultāti liecina, ka 2. tipa cukura diabēta pacientiem NO. un tā metabolītu koncentrācija asinīs ir paaugstināta un tā korelē ar glomerulārās filtrācijas ātrumu, mikroalbumīnūriju un HbA1c%. Diabētiskām žurkām NO. biosintēze ir pastiprināta, bet 1,4-dihidropiridīnu atvasinājumi to samazina. Žurkām ar eksperimentālo cukura diabētu novēro GLUT1 hiperekspresiju nierēs, sirdī un aknās, un nomāktu GLUT4 ekspresiju sirdī un muskuļos, bet mildronāts spēj to normalizēt. Glikozes transportieru ekspresijas un NO. biosintēzes regulācija verētu būt nozīmīga cukura diabēta komplikāciju ārstēšanā. Atslēgvārdi: cukura diabēta komplikācijas, NO., GLUT1, 1,4-dihidropiridīni, mildronāts.
Goals of this thesis were to study the pathogenetical role of changes in nitric oxide metabolism and glucose transporter expression in development of diabetes mellitus and its complications, as well as the ability of mildronate and some 1,4-dihydropyridines to attenuate these changes. The results show that the level of NO. and its metabolites is increased in the blood of diabetic patients and it correlates with glomerular filtration rate, microalbuminuria and HbA1c%. NO. production is increased in diabetic animals, it can be reduced by 1,4-dihydropyridines. In rats with experimental diabetes mellitus GLUT1 expression is increased in kidneys, heart and liver, on the contrary GLUT4 expression is decreased in heart and muscles. Expression of these transporters can be normalized by mildronate. Thus regulation of glucose transporter expression and NO. production could become a novel approach for treatment of diabetic complications. Keywords: diabetic complications, NO., GLUT1, 1,4-dihydropyridines, mildronate
Goals of this thesis were to study the pathogenetical role of changes in nitric oxide metabolism and glucose transporter expression in development of diabetes mellitus and its complications, as well as the ability of mildronate and some 1,4-dihydropyridines to attenuate these changes. The results show that the level of NO. and its metabolites is increased in the blood of diabetic patients and it correlates with glomerular filtration rate, microalbuminuria and HbA1c%. NO. production is increased in diabetic animals, it can be reduced by 1,4-dihydropyridines. In rats with experimental diabetes mellitus GLUT1 expression is increased in kidneys, heart and liver, on the contrary GLUT4 expression is decreased in heart and muscles. Expression of these transporters can be normalized by mildronate. Thus regulation of glucose transporter expression and NO. production could become a novel approach for treatment of diabetic complications. Keywords: diabetic complications, NO., GLUT1, 1,4-dihydropyridines, mildronate
Description
Elektroniskā versija nesatur pielikumus
Keywords
Medicīna , Medicīniskā bioķīmija , Medical biochemistry , cukura diabēta komplikācijas , 1,4-dihidropiridīni , mildronāts , diabetic complications , 1,4-dihydropyridines , mildronate