2-feniletanola biosintēze ar raugiem Kluyveromyces spp. divfāzu ekstraktīvā fermentācijā
Date
2020
Authors
Aksjonova, Lidija
Journal Title
Journal ISSN
Volume Title
Publisher
Latvijas Universitāte
Abstract
Darba mērķis bija izpētīt iespēju veikt laboratorijas mēroga biotehnoloģisku 2-feniletanola (2-PE) producēšanu ar raugiem Kluyveromyces spp. divfāzu ekstraktīvā fermentācijā, izmantojot laktozi saturošas barotnes, tai skaitā pārtikas industrijas atkritumproduktus. No trijiem Kluyveromyces spp. raugiem augstākā 2-PE koncentrācija tika iegūta fermentācijā ar K. marxianus DSM 5422. Divfāzu fermentācijā 2-PE daudzums palielinājās trīs reizes, salīdzinot ar vienfāzu fermentāciju. Maksimālā 2-PE koncentrācija 3,35 g/L tika sasniegta pievienojot 1/7 daļu eļļas no kopējā fermentācijas tilpuma. Secinājām, ka sūkalu permeāts ir piemērots substrāts 2-PE biosintēzei. Galvenās darbā izmantotās metodes: fermentācija laktozi saturošās barotnēs, divfāzu ekstraktīvā fermentācija, L-fenilalanīna enzimātiskā noteikšana, 2-PE, laktozes un etanola noteikšana ar augstefektīvo šķidruma hromatogrāfiju (HPLC).
The aim of this work was to investigate the possibility of laboratory-scale biotechnological production of 2-phenylethanol (2-PE) with yeast Kluyveromyces spp. in two-phase extractive fermentation, using a lactose containing mediums, including food industry waste products. Among three Kluyveromyces spp. yeasts the highest 2-PE concentration was obtained in fermentation with K. marxianus DSM 5422. The amount of 2-PE obtained in the two-phase fermentation increased threefold, compared to the single-phase fermentation. The maximum concentration of 3,35 g/L 2-PE was obtained by adding 1/7 of total fermentation volume of oil. We concluded that whey permeate is a suitable substrate for 2-PE biosynthesis. The main methods used in the work: fermentation on lactose containing mediums, two-phase extractive fermentation, enzymatic determination of L-phenylalanine, determination of 2-PE, lactose and ethanol with HPLC chromatograph.
The aim of this work was to investigate the possibility of laboratory-scale biotechnological production of 2-phenylethanol (2-PE) with yeast Kluyveromyces spp. in two-phase extractive fermentation, using a lactose containing mediums, including food industry waste products. Among three Kluyveromyces spp. yeasts the highest 2-PE concentration was obtained in fermentation with K. marxianus DSM 5422. The amount of 2-PE obtained in the two-phase fermentation increased threefold, compared to the single-phase fermentation. The maximum concentration of 3,35 g/L 2-PE was obtained by adding 1/7 of total fermentation volume of oil. We concluded that whey permeate is a suitable substrate for 2-PE biosynthesis. The main methods used in the work: fermentation on lactose containing mediums, two-phase extractive fermentation, enzymatic determination of L-phenylalanine, determination of 2-PE, lactose and ethanol with HPLC chromatograph.
Description
Keywords
Bioloģija , divfāzu fermentācija , 2-feniletanols , 2-feniletanola biosintēze , Kluyveromyces spp. , sūkalu permeāts