Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments
Date
2013-01
Authors
Ranka, Renate
Petrovskis, Ivars
Sominskaya, Irina
Bogans, Janis
Bruvere, Ruta
Akopjana, Inara
Ose, Velta
Timofejeva, Irena
Brangulis, Kalvis
Pumpens, Pauls
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Virus-like particles (VLPs) are created by the self-assembly of multiple copies of envelope and/or capsid proteins from many viruses, mimicking the conformation of a native virus. Such noninfectious nanostructures are mainly used as antigen-presenting platforms, especially in vaccine research; however, some of them recently were used as scaffolds in biotechnology to produce targeted nanoparticles for intracellular delivery. This study demonstrates the creation of fusion VLPs using hepatitis B core protein-based system maintaining a fibronectin-binding property from B. burgdorferi BBK32 protein, including the evidence of particles’ transmission to BHK-21 target cells via caveolae/rafts endocythosis. These results make this construct to be an attractive model in development of HBc-based nanoparticles for cellular targeting applications and highlights the fragment of B. burgdorferi BBK32 as a novel cellular uptake-promoting peptide.
Description
Keywords
Research Subject Categories::MEDICINE , Research Subject Categories::NATURAL SCIENCES::Biology