Medicīnas un dzīvības zinātņu nozaru galvenās publikācijas

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γ1- and γ2-melanocyte stimulating hormones induce central anxiogenic effects and potentiate ethanol withdrawal responses in the elevated plus-maze test in mice
(Elsevier, 2009) Jansone, Baiba; Rumaks, Juris; Dzirkale, Zane; Pupure, Jolanta; Svirskis, Šimons; Muceniece, Ruta; Klusa, Vija
Little is known about the endogenous functions of γ1- and γ2-melanocyte stimulating hormones (γ1- and γ2-MSH). Although γ-MSHs bind to melanocortin receptor subtypes 3 and 4, we have previously shown that these peptides also influence non-melanocortinergic processes, such as dopaminergic and GABAergic. The aim of this study was to determine the effects of γ1- and γ2-MSH (at doses 0.3, 1 and 2 nmol/mouse/5 μl) on the anxiety levels in mice in elevated plus maze. Three experimental paradigms were performed to assess the effects of peptides on: a) ethanol withdrawal; b) acute ethanol-induced anxiolytic action; c) peptides per se. We used ethanol as the model substance, since its action involves either dopaminergic/GABAergic or melanocortinergic processes. γ-MSHs were administered intracisternally in mice and behavioural responses were assessed in the elevated plus maze test. This study provides the first demonstration of an anxiogenic effect of γ1- and γ2-MSH, their synergistic/additive effect on ethanol withdrawal-induced anxiety behaviour, and an antagonism of peptides involved in the anxiolytic action of ethanol. Furthermore, results suggest that γ-MSHs belong to an anxiogenic peptide family that may play an important role in anxiety disorders as well as in the development of alcohol dependence and/or alcohol withdrawal-induced behaviours.
Can changes in starch content and peroxidase activity be used as rooting phase markers for rhododendron leaf bud cuttings?
(Polish Academy of Sciences Publishing House, 2011) Megre, Dace; Dokane, Kristine; Kondratovics, Uldis
Identification of somatostatin receptor type 5 gene polymorphisms associated with acromegaly
(European Society of Endocrinology, 2011) Ciganoka, Darja; Balcere, Inga; Kapa, Ivo; Peculis, Raitis; Valtere, Andra; Nikitina-Zake, Liene; Lase, Ieva; Schiöth, Helgi B.; Pirags, Valdis; Klovins, Janis
A common promoter variant of the gene encoding cyclooxygenase-1 (PTGS1) is related to decreased incidence ofmyocardial infarction in patients with coronary artery disease
(Elsevier, 2011) Latkovskis, Gustavs; Licis, Normunds; Krivmane, Baiba; Erglis, Andrejs
Cyclooxigenase (COX)-1, formally known as prostanglandin endoperoxideH synthetase-1,mediates synthesis of prostaglandin H2,which is subsequently converted to various biologically active metabolites including thromboxane (TX) A2 [1]. TXA2 is synthesized and released by activated platelets and strongly reinforces thrombus formation, a critical pathway in the pathogenesis of myocardial infarction (MI). Inhibition of COX-1-derived TXA2 in platelets by low-dose aspirin administration reduces incidence of MI [2]. Hypothetically, MI-risk could also be modified by genetic variants that affect activity or expression of COX-1. Many single nucleotide polymorphisms (SNP) in the gene encoding COX-1 (PTGS1) have been described; including functional alterations in both coding and non-coding regions [3,4].We have evaluated if two of such variations are related to the risk ofMI in a historic cohort of patients with coronary artery disease (CAD).
Association of reduced glyoxalase 1 activity and painful peripheral diabetic neuropathy in type 1 and 2 diabetes mellitus patients
(Elsevier, 2013) Skapare, Elina; Konrade, Ilze; Liepinsh, Edgars; Strele, Ieva; Makrecka, Marina; Bierhaus, Angelika; Lejnieks, Aivars; Pirags, Valdis; Dambrova, Maija
Aims: The present study was undertaken to investigate the relationship between glyoxalase 1 (Glo1) enzyme activity and painful diabetic neuropathy (DN) in patients with diabetes mellitus. Methods: Glo1 activity and biochemical markers were determined in blood samples from 108 patients with type 1 diabetes, 109 patients with type 2 diabetes, and 132 individuals without diabetes as a control. Painful and painless peripheral DN was assessed and multivariate regression analysis was used to determine independent association of Glo1 activity with occurrence of painful DN. Results: In patients with type 1 and type 2 diabetes mellitus and painful DN compared to patients with painless DN, Glo1 activity was significantly reduced by 12 and 14%, respectively. The increase in Glo1 activity was significantly associated with reduced occurrence of painful DN after adjusting for confounders by multivariate analysis. Conclusions: Our results demonstrate for the first time that Glo1 activity is lower in patients with both types of diabetes mellitus who were diagnosed with painful DN. These data support the hypothesis that Glo1 activity modulates the phenotype of DN and warrant further investigation into the role of Glo1 in DN. © 2013 Elsevier Inc. All rights reserved.
Lunasin-induced behavioural effects in mice: Focus on the dopaminergic system
(Elsevier, 2013) Dzirkale, Zane; Rumaks, Juris; Svirskis, Simons; Mazina, Olga; Allikalt, Anni; Rinken, Ago; Jekabsons, Kaspars; Muceniece, Ruta; Klusa, Vija
The present study for the first time is devoted to identify central effects of synthetic lunasin, a 43 amino acid peptide. A markedly expressed neuroleptic/cataleptic effect was observed at low (0.1–10 nmol/mouse) centrally administered doses in male C57Bl/6 mice. Lunasin considerably reduced the amphetamine hyperlocomotion but weakly apomorphine climbing behaviour. No influence on ketamine and bicuculline effects was observed. Binding assay studies demonstrated modest affinity of lunasin for the dopamine D1 receptor (Ki = 60±15 M). In a functional assay of cAMP accumulation on live cells lunasin antagonised apomorphine effect on D1 receptor activation (pEC50 = 6.1±0.3), but had no effect in cells expressing D2 receptors. The obtained data suggest that lunasin’s action at least in part is provided via dopaminergic D1 receptor pathways. However, other non-identified mechanisms (probably intracellular) may play an important role in lunasin’s central action. Nevertheless further studies of lunasin are promising, particularly taking into account a necessity for novel type of antipsychotic drugs
Study on the reduction of acrylamide in mixed rye bread by fermentation with bacteriocin-like inhibitory substances producing lactic acid bacteria in combination with Aspergillus niger glucoamylase
(Elsevier, 2013) Bartkiene, Elena; Jakobsone, Ida; Juodeikiene, Grazina; Vidmantiene, Daiva; Pugajeva, Iveta; Bartkevics, Vadims
DNA damage causes TP53-dependent coupling of self-renewal and senescence pathways in embryonal carcinoma cells
(Taylor&Francis, 2013) Jackson, Thomas R.; Salmina, Kristine; Huna, Anda; Inashkina, Inna; Jankevics, Eriks; Riekstina, Una; Kalnina, Zane; Ivanov, Andrey; Townsend, Paul A .; Cragg, Mark S.; Erenpreisa, Jekaterina
Recovering Genetic Resources of Some Legume Species of Latvian Origin by Plant Tissue Culture
(Latvian Academy of Sciences, 2013) Grauda, Dace; Lapiņa, Lita; Jansone, Biruta; Jnsons, Aldis; Rashal, Isaak
Synthesis, physicochemical characterization, cytotoxicity, antimicrobial, anti-inflammatory and psychotropic activity of new N-[1,3-(benzo)thiazol-2-yl]-u-[3,4-dihydroisoquinolin-2(1H)-yl] alkanamides
(Elsevier Masson SAS, 2013) Zablotskaya, Alla; Segal, Izolda; Geronikaki, Athina; Eremkina, Tatiana; Belyakov, Sergey; Petrova, Marina; Shestakova, Irina; Zvejniece, Liga; Nikolajeva, Vizma
Modeling and Query Language for Hospitals
(Springer, 2013) Barzdins, Janis; Barzdins, Juris; Rencis, Edgars; Sostaks, Agris
Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments
(Elsevier, 2013-01) Ranka, Renate; Petrovskis, Ivars; Sominskaya, Irina; Bogans, Janis; Bruvere, Ruta; Akopjana, Inara; Ose, Velta; Timofejeva, Irena; Brangulis, Kalvis; Pumpens, Pauls; Baumanis, Viesturs
Virus-like particles (VLPs) are created by the self-assembly of multiple copies of envelope and/or capsid proteins from many viruses, mimicking the conformation of a native virus. Such noninfectious nanostructures are mainly used as antigen-presenting platforms, especially in vaccine research; however, some of them recently were used as scaffolds in biotechnology to produce targeted nanoparticles for intracellular delivery. This study demonstrates the creation of fusion VLPs using hepatitis B core protein-based system maintaining a fibronectin-binding property from B. burgdorferi BBK32 protein, including the evidence of particles’ transmission to BHK-21 target cells via caveolae/rafts endocythosis. These results make this construct to be an attractive model in development of HBc-based nanoparticles for cellular targeting applications and highlights the fragment of B. burgdorferi BBK32 as a novel cellular uptake-promoting peptide.
Carnitine congener mildronate protects against stress- and haloperidol-induced impairment in memory and brain protein expression in rats
(Elsevier, 2014) Beitnere, Ulrika; Dzirkale, Zane; Isajevs, Sergejs; Rumaks, Juris; Svirskis, Simons; Klusa, Vija
The present study investigates the efficacy of mildronate, a carnitine congener, to protect stress and haloperidol-induced impairment of memory in rats and the expression of brain protein biomarkers involved in synaptic plasticity, such as brain-derived neurotrophic factor (BDNF), acetylcholine esterase and glutamate decarboxylase 67 (GAD67). Two amnesia models were used: 2 h immobilization stress and 3-week haloperidol treatment. Stress caused memory impairment in the passive avoidance test and induced a significant 2-fold BDNF elevation in hippocampal and striatal tissues that was completely inhibited by mildronate. Mildronate decreased the level of GAD67 (but not acetylcholine esterase) expression by stress. Haloperidol decrease by a third hippocampal BDNF and acetylcholine esterase (but not GAD67) expression, which was normalized by mildronate; it also reversed the haloperidol-induced memory impairment in Barnes test. The results suggest the usefulness of mildronate as protector against neuronal disturbances caused by stress or haloperidol.
Primary culture of avian embryonic heart forming region cells to study the regulation of vertebrate early heart morphogenesis by vitamin A
(BioMed Central Ltd, 2014) Cakstina, Ieva; Riekstina, Una; Boroduskis, Martins; Nakurte, Ilva; Ancans, Janis; Zile, Maija H.; Muiznieks, Indrikis
Juvenile Idiopathic Arthritis Subtype- and Sex-specific Associations with Genetic Variants in the PSMA6/PSMC6/PSMA3 Gene Cluster
(Elsevier, 2014) Sjakste, Tatjana; Paramonova, Natalia; Rumba-Rozenfelde, Ingrida; Trapina, Ilva; Sugoka, Olga; Sjakste, Nikolajs
Seasonal dynamics of biomarkers in infaunal clam Macoma balthica from the Gulf of Riga (Baltic Sea)
(Elsevier, 2014) Barda, Ieva; Purina, Ingrida; Rimsa, Elina; Balode, Maija
Copper-Catalyzed Intermolecular C–H Amination of (Hetero)arenes via Transient Unsymmetrical λ3-Iodanes
(ACS, 2014) Sokolovs, Igors; Lubriks, Dmitrijs; Suna, Edgars
Breath testing as a method for detecting lung cancer
(Taylor&Francis, 2014) Taivans, Immanuels; Bukovskis, Maris; Strazda, Gunta; Jurka, Normunds
Early diagnosis of lung cancer is important due to high mortality in late stages of the disease. An ideal approach for population screening could be the breath analysis, due to its non-invasiveness, simplicity and cheapness. Using sensitive methods of analysis like gas chromatography/mass spectrometry in exhaled air of cancer patients were discovered some volatile organic compounds – possible candidates for cancer markers. However, these compounds were not specific for cancer cells. At the same time, integrative approaches used to analyze the exhaled breath have demonstrated high sensitivity and specificity of this method for lung cancer diagnosis. Such integrative approaches include detection of smell prints by electronic nose or integrated analysis of wide range of volatile organic compounds detected by gas chromatography/mass spectrometry or related methods. Modern statistical pattern recognition systems like logistic regression analysis, support vector machine or analysis by artificial neuronal network may improve diagnostic accuracy.
Sediment quality of the ecoregion Engure, Gulf of Riga, assessed by using ecotoxicity tests and biomarker responses
(Latvian Academy of Sciences, 2014) Putna, Ieva; Strode, Evita; Bārda, Ieva; Puriņa, Ingrīda; Rimša, Elīna; Balode, Maija
Does shoot anatomical heterogeneity influence Ex Vitro adventitious root formation in rhododendron microcutting?
(SEJANI Ltd., 2014) Dokane, Kristine; Megre, Dace; Lazdane, Madara; Kondratovics, Uldis

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